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doi: 10.1016/j.jtemb.2013.07.005 253 SharmaGSharmaARBhaveshRParkJGanboldBNamJSet al

Diabetic and hypertensive retinopathy therapy with anthocyanosides: double blind placebo-controlled clinical trial

However, melatonin is not exclusively produced in the pineal gland, but it is also locally synthesized in several cells and tissues, such as the retina, the gastrointestinal tract, and the innate immune system 10

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Roshanbin et al., 2016

Both urea and guanidinium chloride are powerful denaturants and would unfold the protein, if the protein binds to the resin in presence of these denaturants, it can be said with certainty that folded protein hides the his-tag and prevents its binding to the IMAC resin